Abstract
The Gram-positive, spore-forming bacterium, Clostridioides difficile is the leading cause of healthcare-associated infections in the United States, although
it also causes a significant number of community-acquired infections. C. difficile infections, which range in severity from mild diarrhea to toxic megacolon, cost more
to treat than matched infections, with an annual treatment cost of approximately $6
billion for almost half-a-million infections. These high–treatment costs are due to
the high rates of C. difficile disease recurrence (>20%) and necessity for special disinfection measures. These
complications arise in part because C. difficile makes metabolically dormant spores, which are the major infectious particle of this
obligate anaerobe. These seemingly inanimate life forms are inert to antibiotics,
resistant to commonly used disinfectants, readily disseminated, and capable of surviving
in the environment for a long period of time. However, upon sensing specific bile
salts in the vertebrate gut, C. difficile spores transform back into the vegetative cells that are responsible for causing
disease. This review discusses how spores are ideal vectors for disease transmission
and how antibiotics modulate this process. We also describe the resistance properties
of spores and how they create challenges eradicating spores, as well as promote their
spread. Lastly, environmental reservoirs of C. difficile spores and strategies for destroying them particularly in health care environments
will be discussed.
Keywords
Clostridioides difficile
- spore - germination - disinfection - resistance
